《植物生理学报》 2009, 45(9): 865-868

采用RAPD 技术探讨花椒的遗传多样性及其与环境的关系

李庆芝*, 刘振伟, 毕于义, 李承永, 李玲

莱芜市农业科学研究院, 山东莱芜271100

通信作者：李庆芝；E-mail: qingzhili123@sohu.com；Tel: 0634-8867570

摘　要：

采用 RAPD 分子标记方法分析 11 个花椒品种的遗传多样性, 以及遗传多样性与环境因素的相关性。从 60 条随机引 物中筛选出 10 条引物, 共扩增出 67 条带, 平均每个引物扩增出 6.7 条, 其中 56 条具有多态性, 多态比例为 84%。根据品种 间的遗传距离构建的聚类分析树状图, 11 个花椒品种的相似系数在0.08~0.92 之间, 可分为2个类群, 这种分类与花椒的叶 的外形分类结果一致。不同地域种植的同一品种材料遗传距离较大, 显示花椒的遗传多样性与地域分布有关系。

关键词：花椒; RAPD; 遗传多样性; 聚类分析

收稿：2009-06-24   修定：2009-08-26

资助：山东省农业良种工程项目。

Exploring Genetic Diversity and Its Relations to Environmental Factors in Zanthoxylum bungeanum Maxim. by RAPD Technique

LI Qing-Zhi*, LIU Zhen-Wei, BI Yu-Yi, LI Cheng-Yong, LI Ling

Laiwu Agriculture Academic Research Institute, Laiwu, Shangdong 271100, China

Corresponding author: LI Qing-Zhi; E-mail: qingzhili123@sohu.com; Tel: 0634-8867570

Abstract:

Randomly amplified polymorphic DNA (RAPD) was used to analyze the genetic diversity and its relations with environmental factors in 11 cultivars of Zanthoxylum bungeanum Maxim.. Ten primers were screened out of 60 random primers. A total of 67 detectable fragments were amplified with an average of 6.7 bands per primer. Fifty-six fragments were polymorphic and the percentage was about 84%. We also used the result of RAPD to assess the genetic distance and to perform clustering analysis. The genetic similarity coefficient was 0.08–0.92 among 11 cultivars of Z. bungeanum. The 11 cultivars of Z. bungeanum could be divided into two major clusters and this taxonomy was consistent with the one according to leaf morphology. The abundant genetic differentiation of Z. bungeanum in different regions indicated the genetic variation of Z. bungeanum was related with regional differences.

Key words: Zanthoxylum bungeanum Maxim.; randomly amplified polymorphic DNA (RAPD); genetic diversity; clustering analysis